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NNP1 Antibody / Novel nuclear protein 1 / RRP1

Ribosomal RNA processing protein 1 homolog A, also called Novel nuclear protein 1 (NNP1), is a protein that in humans is encoded by the RRP1 gene. The protein encoded by this gene is the putative homolog of the yeast ribosomal RNA processing protein RRP1. The encoded protein is involved in the late stages of nucleologenesis at the end of mitosis, and may be required for the generation of 28S rRNA.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P56182

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids Q22-D237) was used as the immunogen for the NNP1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NNP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NNP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NNP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NNP1 antibody should be determined by the researcher.

Location

Nucleus, Nucleolus

Image Legend

Immunofluorescent staining of FFPE human U-87 MG cells with NPP1 antibody (red), Beta Tubulin mAb (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
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