MRNA-capping enzyme Antibody / RNGTT / HCE

MRNA-capping enzyme is a protein that in humans is encoded by the RNGTT gene. Enables mRNA guanylyltransferase activity and triphosphatase activity. Involved in 7-methylguanosine mRNA capping. Predicted to be located in nucleoplasm.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O60942

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids L11-D438) was used as the immunogen for the mRNA-capping enzyme antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the mRNA-capping enzyme antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This mRNA-capping enzyme antibody is available for research use only.

Storage Conditions

After reconstitution, the mRNA-capping enzyme antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the mRNA-capping enzyme antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human intestine diffuse large B-cell lymphoma tissue with mRNA-capping enzyme antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.