RBM25 Antibody / RNA-binding protein 25

RNA-binding protein 25, also called Arg/Glu/Asp-rich protein of 120 kDa (RED120), is a protein that in humans is encoded by the RBM25 gene. Enables mRNA binding activity. Involved in regulation of alternative mRNA splicing, via spliceosome and regulation of apoptotic process. Located in nuclear speck.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P49756

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids D100-E820) was used as the immunogen for the RBM25 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the RBM25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RBM25 antibody is available for research use only.

Storage Conditions

After reconstitution, the RBM25 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RBM25 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear specks

Image Legend

Immunofluorescent staining of FFPE human U-2 OS cells with RBM25 antibody (green), Alpha Tubulin mAb (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.