EZH1 Antibody / Enhancer of zeste homolog 1

Histone-lysine N-methyltransferase EZH1 is an enzyme that in humans is encoded by the EZH1 gene. EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q92800

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R27-E485) was used as the immunogen for the EZH1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the EZH1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This EZH1 antibody is available for research use only.

Storage Conditions

After reconstitution, the EZH1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the EZH1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of 1) rat testis, 2) rat C6, 3) mouse testis and 4) mouse NIH 3T3 cell lysate with EZH1 antibody. Predicted molecular weight: 69-86 kDa (multiple isoforms) .