U1 snRNP C Antibody / SNRPC

U1 small nuclear ribonucleoprotein C is a protein that in humans is encoded by the SNRPC gene. This gene encodes one of the specific protein components of the U1 small nuclear ribonucleoprotein (snRNP) particle required for the formation of the spliceosome. The encoded protein participates in the processing of nuclear precursor messenger RNA splicing. snRNP particles are attacked by autoantibodies frequently produced by patients with connective tissue diseases. The genome contains several pseudogenes of this functional gene. Alternative splicing results in a non-coding transcript variant.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P09234

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids M1-R159) was used as the immunogen for the U1 snRNP C antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the U1 snRNP C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This U1 snRNP C antibody is available for research use only.

Storage Conditions

After reconstitution, the U1 snRNP C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the U1 snRNP C antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human prostate adenocarcinoma tissue with U1 snRNP C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.