Crk-like protein Antibody / CrkL

Crk-like protein is a protein that in humans is encoded by the CRKL gene. This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

UniProt

P46109

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids M1-H290) was used as the immunogen for the Crk-like protein antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IP, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Crk-like protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Crk-like protein antibody is available for research use only.

Storage Conditions

After reconstitution, the Crk-like protein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Crk-like protein antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Immunofluorescent staining of FFPE human U-87 MG cells with Crk-like protein antibody (red) and Alpha Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.