CHGA Antibody / Chromogranin A

CHGA, also known as CGA or Chromogranin A, is an acidic protein co-stored and co-released with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P10645

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids L19-E417) was used as the immunogen for the CHGA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CHGA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CHGA antibody is available for research use only.

Storage Conditions

After reconstitution, the CHGA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CHGA antibody should be determined by the researcher.

Location

Finely granular cytoplasmic

Image Legend

IHC staining of FFPE human lung adenocarcinoma tissue with CHGA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.