MSin3A Antibody / SIN3A

Paired amphipathic helix protein Sin3a is a protein that in humans is encoded by the SIN3A gene. The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96ST3

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids Q8-E918) was used as the immunogen for the mSin3A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the mSin3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This mSin3A antibody is available for research use only.

Storage Conditions

After reconstitution, the mSin3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the mSin3A antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human ovarian cancer tissue with mSin3A antibody (red) and DAPI (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.