Lamin A/C Antibody

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A, B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

P02545

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids Y481-Y646) was used as the immunogen for the Lamin A/C antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

5F3C12

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Lamin A/C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Lamin A/C antibody is available for research use only.

Storage Conditions

After reconstitution, the Lamin A/C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Lamin A/C antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Lamin A/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.