HDKIR Antibody / C3IP1 / KLHL12

Kelch-like protein 12, also called CUL3-interacting protein 1 (C3IP1) and DKIR homolog (hDKIR), is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

UniProt

Q53G59

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R27-I331) was used as the immunogen for the hDKIR antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

2G11D1

Applications

WB, IHC-P

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the hDKIR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This hDKIR antibody is available for research use only.

Storage Conditions

After reconstitution, the hDKIR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the hDKIR antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human thyroid cancer tissue with hDKIR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.