SF3a60 Antibody / SAP 61 / Splicing factor 3A subunit 3

Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene. This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2ug per 500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q12874

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids E123-Q472) was used as the immunogen for the SF3a60 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, IP, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SF3a60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SF3a60 antibody is available for research use only.

Storage Conditions

After reconstitution, the SF3a60 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SF3a60 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human ovarian serous cancer tissue with SF3a60 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.