SF3a120 Antibody / Splicing factor 3A subunit 1 / SF3A1

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP) . U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q15459

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids K20-Q556) was used as the immunogen for the SF3a120 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, IP, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SF3a120 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SF3a120 antibody is available for research use only.

Storage Conditions

After reconstitution, the SF3a120 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SF3a120 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of 1) rat lung, 2) rat brain, 3) rat thymus, 4) rat C6, 5) mouse lung, 6) mouse brain, 7) mouse thymus and 8) mouse Neuro-2a cell lysate with SF3a120 antibody. Predicted molecular weight ~89 kDa but commonly observed at ~120 kDa.