Leukocyte elastase inhibitor Antibody / LEI / SERPINB1 / PI-2

Leukocyte elastase inhibitor (LEI) also known as Serpin B1 and Peptidase inhibitor 2 (PI-2) is a protein that in humans is encoded by the SERPINB1 gene. The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P30740

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids A52-R275) was used as the immunogen for the Leukocyte elastase inhibitor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Leukocyte elastase inhibitor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Leukocyte elastase inhibitor antibody is available for research use only.

Storage Conditions

After reconstitution, the Leukocyte elastase inhibitor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Leukocyte elastase inhibitor antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human A549 cells with Leukocyte elastase inhibitor antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.