Toll-interacting protein Antibody / TOLLIP

TOLLIP (TOLL-Interacting Protein) is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al. (2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By western blot analysis of embryonic kidney cells, Burns et al. (2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al. (2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9H0E2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids M1-P274) was used as the immunogen for the Toll-interacting protein antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Toll-interacting protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Toll-interacting protein antibody is available for research use only.

Storage Conditions

After reconstitution, the Toll-interacting protein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Toll-interacting protein antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of FFPE human A549 cells with Toll-interacting protein antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.