C-terminal-binding protein 2 Antibody / CTBP2

The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

P56545

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids H321-Q445) was used as the immunogen for the C-terminal-binding protein 2 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

7F3E1

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the C-terminal-binding protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This C-terminal-binding protein 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the C-terminal-binding protein 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the C-terminal-binding protein 2 antibody should be determined by the researcher.

Location

Nucleus, Cell junction

Image Legend

IHC staining of FFPE mouse colon tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.