TRPC7 Antibody / Short transient receptor potential channel 7

Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a human gene encoding a protein of the same name. Using a cosmid/BAC contig, this gene is mapped to chromosome 21q22.3. TRPC7 is the most abundantly expressed long TRPC in peripheral blood and in some blood cell lines. The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9HCX4

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R489-I862) was used as the immunogen for the TRPC7 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TRPC7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRPC7 antibody is available for research use only.

Storage Conditions

After reconstitution, the TRPC7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRPC7 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human U-251, 4) human SK-N-SH and 5) rat C6 cell lysate with TRPC7 antibody. Expected molecular weight ~100 kDa.