Dnmt3a Antibody

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000-1:4000

UniProt

Q9Y6K1

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Dnmt3a was used as the immunogen for the Dnmt3a antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1823CT281.43.36

Applications

Purity

Protein G purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Dnmt3a antibody is available for research use only.

Storage Conditions

Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Dnmt3a antibody at 1:2000: Lane 1) human T47D, 2) CCRF-CEM, 3) Jurkat and 4) HeLa cell lysate. Predicted molecular weight: 100-130 kDa.