SMURF2 Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000-1:4000

UniProt

Q9HAU4

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human SMURF2 was used as the immunogen for the SMURF2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1826CT865.51.28

Applications

Purity

Protein G purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SMURF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMURF2 antibody is available for research use only.

Storage Conditions

Aliquot the SMURF2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SMURF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.