HACE1 Antibody

HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is an E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000

UniProt

Q8IYU2

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human HACE1 was used as the immunogen for the HACE1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1854CT757.66.47

Applications

Purity

Protein G purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HACE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HACE1 antibody is available for research use only.

Storage Conditions

Aliquot the HACE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HACE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.