HACE1 Antibody
HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 is an E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:2000
UniProt
Q8IYU2
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human HACE1 was used as the immunogen for the HACE1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1854CT757.66.47
Applications
WB
Purity
Protein G purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HACE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HACE1 antibody is available for research use only.
Storage Conditions
Aliquot the HACE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
Western blot testing of HACE1 antibody at 1:2000: Lane 1) human HEK293 and 2) 293T/17 cell lysate. Predicted molecular weight ~102 kDa.
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