Wee1 Antibody
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:2000
UniProt
P30291
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 96-130 from human Wee1 was used as the immunogen for the Wee1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Wee1 antibody is available for research use only.
Storage Conditions
Aliquot the Wee1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
Western blot testing of Wee1 antibody at 1:2000: Lane 1) human HeLa, 2) Jurkat and 3) K562 cell lysate. Predicted molecular weight ~72 kDa.
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