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CH25H Antibody (N-Terminal Region)

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:2000

UniProt

O95992

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 47-76 from human CH25H was used as the immunogen for the CH25H antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CH25H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CH25H antibody is available for research use only.

Storage Conditions

Aliquot the CH25H antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CH25H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Pig

Image Legend

Western blot testing of CH25H antibody at 1:1000: Lane 1) human brain lysate, 2) mouse heart lysate and 3) mouse lung lysate. Predicted molecular weight ~32 kDa.

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