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MYPT1 Antibody / Myosin Phosphatase / PPP1R12A

Key regulator of protein phosphatase 1C (PPP1C) . Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:4000

UniProt

O14974

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 641-674 from human MYPT1 was used as the immunogen for the MYPT1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MYPT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MYPT1 antibody is available for research use only.

Storage Conditions

Aliquot the MYPT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MYPT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Rat

Image Legend

Western blot testing of MYPT1 antibody at 1:4000: Lane 1) human HeLa, 2) human MCF-7, 3) mouse C2C12 and 4) mouse NIH3T3 cell lysate. Predicted molecular weight ~115 kDa.

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