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Myosin Phosphatase Antibody / MYPT1 / PPP1R12A

Key regulator of protein phosphatase 1C (PPP1C) . Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:4000

UniProt

O14974

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 481-514 from the human MYPT1 was used as the immunogen for the Myosin Phosphatase antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Myosin Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Myosin Phosphatase antibody is available for research use only.

Storage Conditions

Aliquot the Myosin Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Myosin Phosphatase antibody at 1:4000: Lane 1) human HEK293, 2) (h) HeLa, 3) (h) HT-29, 4) (h) MCF-7, 5) mouse C2C12, 6) (m) NIH3T3 and 7) (m) uterus lysate. Predicted molecular weight ~115 kDa.

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