Mouse monoclonal antibody to HIV-1 Nef

3'ORF

Reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HXB2)

Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli)

Monoclonal

3D12

Unconjugated

Infectious Disease & Virology

Protein G purification

ELISA 0.1 to 0.2 μg/ml; WB 2 μg/ml. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

ELISA, ICC, WB

Mouse

PBS pH 7.4, with 0.1% sodium azide

IgG1