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Chicken polyclonal antibody to human GFRa-3

Chicken polyclonal antibody to human GFRa-3

Product Specifications

Product Name Alternative

GDNF receptor alpha-3

UniProt

O60609

Cross Reactivity

Human GFRa-3, doesn't cross-react with hGFRa-1, hGFRa-2, hGFRa-4

Immunogen

Recombinant His-tagged human GFRa-3 protein produced using CHO-based suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

GFRα-3 affinity purified

Dilution

WB 1 to 2 μg/ml; ELISA: 3 to 6.25 ng/ml; IF 0.3 to 0.67 μg/ml. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, IF, WB

Host or Source

Gallus

Preservative

Concentrated ammonium sulphate in PBS pH 7.4

Isotype

IgY

Available Sizes

Frequently Asked Questions

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