What is the catalog number for PECAM-1, Mouse, mAb MEC7.46 Antibody?

The catalog number for PECAM-1, Mouse, mAb MEC7.46 Antibody is orb1109192.

What sizes are available for PECAM-1, Mouse, mAb MEC7.46 Antibody?

PECAM-1, Mouse, mAb MEC7.46 Antibody is available in the following size(s): 100 µg.

Is PECAM-1, Mouse, mAb MEC7.46 Antibody in stock?

PECAM-1, Mouse, mAb MEC7.46 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

PECAM-1, Mouse, mAb MEC7.46 Antibody

Mouse monoclonal antibody to PECAM-1

Product Specifications

Product Name Alternative

CD31, platelet-endothelial cell adhesion molecule-1 Gene name: Pecam1

UniProt

Q08481

Reactivity

Mouse

Immunogen

Mouse t.end.1 cells (polyoma middle T (PmT) -transformed EC)

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1) F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1) . P: Serial paraffin-embedded 4 um sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3) . IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa.