CD34, Mouse, mAb MEC14.7 Antibody
Mouse monoclonal antibody to CD34
Product Specifications
Product Name Alternative
Hematopoietic progenitor cell antigen CD34
UniProt
Q64314
Reactivity
Mouse
Cross Reactivity
Human
Immunogen
Murine transformed EC t-end.1
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: both reduced/non-reduced; 70-105 kDa, depending on glycosylation state and cell type (Ref1) P: Formalin fixed; boiled twice for 5 minutes in citrate (pH 6.0) as antigen retrieval (in house tested; Ref 4,6) or 0.03 % trypsin treatment for 10 minutes at 37°C (Ref3) FC: mice blood cells; 0.1ug MEC14.7/106 cells (Ref5) IP: ~100 kDa protein in H5V cells (Ref1) CD34 in mouse glioma. Staining of paraffin tissue section with antibody MEC14.7. Anti-mouse CD34 at 5μg/ml (o/n, 4°C) resulted in the specific staining of endothelial cells.
Tested Applications
FC, IF, IHC-Fr, IP, PS, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
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