TLR4/MD-2, Mouse, mAb MTS510 Antibody
Mouse monoclonal antibody to TLR4/MD-2
Product Specifications
Product Name Alternative
Toll-like receptor 4:- TLR4, CD284, ARMD10 MD2: ESOP-1; myeloid differentiation protein-2; lymphocyte antigen 96 (Ly-96)
Reactivity
Mouse
Immunogen
Ba/F3 cells expressing mouse TLR4 and MD-22
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS and 0.1% bovine serum albumin.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
F: 6 μm acetone fixed sections blocked with 0.3% hydrogen peroxidase in methanol and subsequently with normal serum. Sections were incubated for 2h at RT at 1/100 dilution of mAb Fc: Cells were incubated with 0.1um mAb for 30 minutes at 4°C FS: Cells were pre-incubated with 10 μg/ml of antagonistic mAb MTS510 RAW264.7 cells (105) were stained with 4μg/ml mAb for 1h at 4°C.
Tested Applications
FC, Functional Studies, IF, IHC-Fr, IP
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Rat IgG2a
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