MBL-C, Mouse, mAb 14D12 Antibody

For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium-dependent antibody) .

F: Cryostat sections (7um) of mouse tissue were acetone fixed and stained o/n at 4°C in TBS/2% goat serum. (Ref.4) IA: Biotinylated 14D12 was used as detector in ELISA at 1μg/ml TBS/Tween-20/Ca (Ref.1) IF: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12. (Ref.3) W: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50Kda is detected. Under reducing conditions a 26 KDa band is detected. (Ref.2) MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading macrophages invading the intima (insert) . MBL-C bound, similar to MBL-A, at sites of necrosis (upper right corner) . No MBL-C binding was shown in the media or on fibrous caps covering the thickened intima.