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C3, Mouse, mAb 11H9 Antibody

Mouse monoclonal antibody to C3

Product Specifications

Product Name Alternative

Omplement component C3, HSE-MSF Gene name: C3

Reactivity

Mouse

Immunogen

C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For Western blotting, flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IA: Antibody 11H9 was used as coating 0.5 μg/well in PBS. W: A- reduced sample treatment and SDS-PAGE was used. The band sizes are ~110-115 kDa (; -chain), ~80 and 70 kDa (Ref.1) . F:- Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution (Ref 1, 3) . IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um; m. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval. (Ref.3) .

Tested Applications

FC, IA, IF, IHC-Fr, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Isotype

Rat IgG2a

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