What is the catalog number for C4, Mouse, mAb 16D2 Antibody?

The catalog number for C4, Mouse, mAb 16D2 Antibody is orb1109157.

What sizes are available for C4, Mouse, mAb 16D2 Antibody?

C4, Mouse, mAb 16D2 Antibody is available in the following size(s): 100 µg.

Is C4, Mouse, mAb 16D2 Antibody in stock?

C4, Mouse, mAb 16D2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

C4, Mouse, mAb 16D2 Antibody

Mouse monoclonal antibody to C4

Product Specifications

Product Name Alternative

Complement component 4

Reactivity

Mouse

Immunogen

Thymocytes decorated with Thy-1 antibody and complement components

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For Immuno Assays, Immuno Fluorescence, Immuno Precipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

W: A non-reduced sample treatment and SDS-Page was used. The band size is 200 kDa (Ref.4) . IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20 (Ref.2) . IF: Spleens were snap frozen and stored at -80°C until cryosections were cut. Sections (5 μm) were fixed for 4 min in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS, and PBS. Biotinylated 16D2 was visualized using avidin-PE (BD PharMingen, San Diego, CA) (Ref.2) .