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Nectin-3, Mouse, mAb 103-A1 Antibody

Mouse monoclonal antibody to Nectin-3

Product Specifications

Product Name Alternative

PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113

Reactivity

Mouse

Cross Reactivity

Human - No, Rat - No

Immunogen

Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Dilution

For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IF: fixed in 2% paraformaldehyde in PBS optionally followed by methanol/aceton fixation (Ref 2-7) FC: 1 ug per 1 x 106 cells FS: injection of 1ug into lumen of seminiferous tubules (Ref 7) IA: Indirect ELISA on 0.5μg/ml mouse nectin-3 coated per well. Positive signal with 2μg/ml antibody. IF: Cells are fixed in 2 % paraformaldehyde in PBS, optionally followed by methanol/aceton fixation (Ref 1-6) IP: 1-2 ug per 100-500 ug of total protein (1 ml of cell lysate)

Tested Applications

FC, Functional Studies, IA, IF, IHC-Fr, IP

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide

Isotype

Rat IgG2a

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