TLR2, Mouse, mAb T2.5 Antibody
Mouse monoclonal antibody to TLR2
Product Specifications
Product Name Alternative
TLR2, CD282, TIL4 Gene name: Tlr2
Reactivity
Human, Mouse
Cross Reactivity
Human - Yes
Immunogen
Mouse TLR2 peptide
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IHC-F: 6um sections were fixed with aceton. Sections were blocked with goat serum and exposed o/n with T2.5. FC: 4*104 leukocytes/ml were stained for 30 minutes at 4°C. FS: mice were injected i.p. with 1 mg T2.5, after 1h incubation mice were challenged; T2.5 5 μg/ml was added to cell culture. IP: 40ug cleared protein was incubated with 2ug T2.5 for 1h at 4°C. IA: T2.5 as a detector.
Tested Applications
FC, Functional Studies, IA, IF, IHC-Fr, IP
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG1
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