Macrophages F4/80, Mouse, mAb BM8 Antibody
Mouse monoclonal antibody to Macrophages F4/80
Product Specifications
Reactivity
Mouse
Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1) ; reduction with 2-mercaptoethanol destroys BM8 antigen. F: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control. P: fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control (Ref 3) . FC: fixed with 1 % paraformaldehyde (Ref 1) .
Tested Applications
FC, IHC-Fr, PS, WB
Preservative
1 ml (100 μg/ml) 0.2 μm filtered in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
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