C5aR, Mouse, mAb 20/70 Antibody
Mouse monoclonal antibody to C5aR
Product Specifications
Product Name Alternative
C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R Gene name: C5ar1
Reactivity
Mouse
Immunogen
RBL-2H3 transfectantsexpressing murine C5aR on the cell surface.
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
Form
1 ml (100 μg/ml) 0.2 um protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
FC: 2x10^5 cells were blocked with human and murine IgG on ice for 20€. Cells were stained with 10ug antibody 20/70 for 45€ in PBS/1.5%FCS. For analysis with FACS cells were washed with PBS/1%formaldehyde (Ref.2) . FS: Before migration towards C5a was induced, 2x105 cells were incubated for 30€ with 10ug antibody 20/70 in a transwell migration assay (Ref.2) . IF: Cells were cultured on coverslips, fixed with 4% PFA for 20€, blocked with 3% BSA for 2h and stained with antibody 20/70 for 2h (Ref.6) .
Tested Applications
FC, Functional Studies, IF
Preservative
0.2 ml (100 μg/ml) 0.2 μm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.
Isotype
Rat IgG2b
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