VAP-1, Mouse, mAb 7-88 Antibody

For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting.

FC: Antibody 7-88 stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 cDNA. . As positive control- anti-VAP-1 clone 7-106 was used and as negative control an isotype-matched control antibody. (Ref. 2) IHC-F:- Tissue sections were fixed in acetone and incubated with antibody 7-88 for 20 minutes at room temperature. . As negative control an irrelevant isotype-matched antibody was used (Ref.2) . FS: Antibody 7-88 (200um) was intravenously injected which resulted in the inhibition of leukocyte trafficking in inflamed peritoneum.- (Ref.2) .