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C1q, Mouse, mAb JL-1 Antibody

Mouse monoclonal antibody to C1q

Product Specifications

Product Name Alternative

Complement component C1q, Complement C1q subcomponent subunit A

Reactivity

Mouse

Cross Reactivity

Human - Yes, Rat - Yes

Immunogen

Purified mouse C1q

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC-F: Antibody JL-1 was used to stain tissue sections which were fixed in acetone. As positive control a polyclonal anti-C1q antibody was used and as negative control an isotype matched monoclonal antibody (Ref.1) . FS: Antibody JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1) .

Tested Applications

Functional Studies, IA, IF, IHC-Fr, WB

Preservative

Quantities >500 μg will be sent without preservatives and are carrier-free.

Isotype

Mouse IgG2b

Frequently Asked Questions

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