CD200R3, Mouse, mAb Ba103 Antibody

For flow cytometry, Western blotting and immuno precipitation, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

WB: reduced and non-reduced MC/9 lysate; band size 38kDa under reducing conditions and 74 kDa under nonreducing conditions FC: Basophils and mast cells; no fixation needed FS: antagonist in basophil-mediated allergic reactions; a single intravenous administration of 30 um antibody . The biological activity of the antibody is drastic reduction in the basophile number in the peripheral blood and spleen, to approximately 20% of the normal level; also useful for mast cell degranulation; readout by measuring hexosaminidase activity IP: mast cells were lysed with 1% NP-40 containing lysis buffer with proteinase inhibitor mixture before incubation with the antibody