C3aR, Mouse, mAb 14D4 Antibody
Mouse monoclonal antibody to C3aR
Product Specifications
UniProt
O09047
Reactivity
Mouse
Immunogen
RBL-2H3 transfectants expressing mouse C3aR
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
FC: Antibody 14D4 stains the extracellular domain of C3aR when RBL-2H3 transfectants and human monocytes are used. Primary antibody concentrations were 5μg/ml. (Ref.1) IHC-F: Tissue sections were fixed in acetone. To block unspecific binding of Abs, sections were incubated with PBS and heat aggregated human IgG at 300 μg/ml (Ref.1) .
Tested Applications
FC, IHC-Fr
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Rat IgG2a
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