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Pentraxin 3, Mouse, mAb 2C3 Antibody

Mouse monoclonal antibody to Pentraxin 3

Product Specifications

UniProt

P48759

Reactivity

Mouse

Immunogen

Recombinant mouse PTX3

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For flow cytometry, immuno assay, immuno fluorescence, immuno precipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

FC: Antibody 2C3 stains the intra and extracellular domain of Pentraxin 3. For intracellular staining cells were permeabilized with buffer containing triton. As positive control transfected cells were used. W: A non-reduced sample treatment and SDS-Page was used. The band size is 400 kDa.

Tested Applications

FC, Functional Studies, IA, IF, IP, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Isotype

Mouse IgG1

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