TNF-RII, Human, mAb MR2-1, FITC Antibody
Mouse monoclonal antibody against TNF-RII conjugated to FITC
Product Specifications
Reactivity
Human
Cross Reactivity
Cynomolgus monkey - Yes, Rhesus monkey - Yes
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Advised positive controls for frozen sections are human lymphnodes and for flow cytometry PHA activated T cells.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IA: It can be used as a capture antibody. IP: The lysate was centrifuged and the supernatant was incubated with 5 mg TNFR2-specific antibody MR2-1 for 2 hours at 4°C. The immuno complexes were precipitated with protein G agarose for two hours at 4°C (Ref.4) . W: A reduced protocol was used. The expected band size is ~65 kDa.
Tested Applications
FC, Functional Studies, IA, IF, IP, WB
Preservative
20 um filtered in PBS+1%rAlbumin+0.02%NaN3
Isotype
Mouse IgG1
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