H-FABP, Human, mAb 66E2 Antibody
Mouse monoclonal antibody to H-FABP
Product Specifications
Product Name Alternative
Heart-type fatty acid-binding protein, Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor Gene name: FABP3, FABP11, MDGI
Reactivity
Human
Cross Reactivity
Mouse - Yes, Rat - Yes, Swine - Yes
Immunogen
Purified human H-FABP
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IP: Biotinylated 66E2 was immobilized on streptavidin beads and added to serum to immunoprecipitate H-FABP (Ref.5) . W: Reduced sample treatment. The band size is ~15 kDa (Ref.4) . 1μg/ml was used (Ref.6) . F: Permeabilization was done in cold acetone with 0.5% hydrogen peroxidase for 10 min, after drying and washing, antibodies were incubated for 30 minutes.
Tested Applications
IA, IHC-Fr, IP, WB
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG1
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