VE-Cadherin, Human, mAb BV9 Antibody

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.

F: Acetone fixed sections were blocked with horse serum and incubated with antibody BV9 for 30 minutes (Ref.2) . FC: Antibody BV9 stains the extracellular domain of VE-cadherin. As negative control an IgG isotype control was used (Ref.4) FS: Antibody BV9 functions as an antagonist. The antibody was functionally tested by adding 10-50μg/ml antibody BV9 to cell culture. The antibody blocks VE-cadherin causing a redistribution of VE-cadherin away from intracellular junctions (Ref.5, 6) . IA: Antibody BV9 can function as coat and detector. IF: Cells on coverslips were fixed with 3% paraformaldehyde and permeabilized with 0.5% Triton X-100 before incubation with antibody BV9 (Ref.5, 8) . W: A reduced sample treatment and 7.5% SDS-Page was used. The band size is 130-140kDa (Ref.3) .

0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.