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Beta1 Integrin, Human, mAb BV7 Antibody

Mouse monoclonal antibody to Beta1 Integrin

Product Specifications

Product Name Alternative

CD29, fibronectin receptor subunit beta, -integrin VLA-4 subunit beta

UniProt

P05556

Reactivity

Human

Immunogen

Human umbilical vein EC

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of beta1 integrin to be inactivated.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

FC: Antibody BV7 stains the extracellular domain of beta-1 integrin. HUVEC cells were fixed in 4% paraformaldehyde before staining. Mouse IgG1 isotype was used as negative. FS: Antibody BV7 functions as an inhibiting antibody. The antibody was functionally tested by adhesion assay (ref 1), chemotaxis (ref 2), patch clamp analysis (ref 3) and neurotoxicity induced apoptosis (ref 4) .

Tested Applications

FC, Functional Studies, IA, IP, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Isotype

Mouse IgG1

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