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Beta-3 integrin subunit, Human, mAb BV4 Antibody

Mouse monoclonal antibody to Beta-3 integrin subunit

Product Specifications

Product Name Alternative

Platelet membrane glycoprotein IIIa, GPIIIa

Reactivity

Human

Cross Reactivity

Bovine - Yes

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Form

1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC-F:- Tissue sections were fixed in acetone. As negative control an irrelevant mouse IgG was used (Ref.2) . FS: Antibody clone BV4 inhibits the downstream activation, reducing the mitogenic effects of two of its ligands, VEGFR-2 and HIV-1-Tat. (Ref.1) . IP:clone BV4 precipitates beta3 integrin complexes from endothelial cell lysate. IHC-P: Tissue sections were deparaffinized in xylene and pretreated with 10mmol/l sodium citrate buffer, pH 6.0

Tested Applications

Functional Studies, IA, IHC-Fr, IP, PS, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.

Isotype

Mouse IgG1

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