Lipoteichoic Acid, mAb 55 Antibody
Mouse monoclonal antibody to Lipoteichoic acid
Product Specifications
Product Name Alternative
LTA
Cross Reactivity
Baboon|Canine|Human|Rat|Swine
Immunogen
Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref. 1)
Clonality
Monoclonal
Conjugation
Unconjugated
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (> 200 μg/ml) culture medium with a low endotoxin level containing 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IA: For detection, 1.2 μg/ml antibody in PBS was added for 1 hr at 37 °C on LTA coated plates (Ref. 2,3,8) . IF: 60€in PBS/0.02%BSA/0.02% saponin (ref 4) W: A reduced or native sample treatment and run on 15% SDS-Page. Blot was incubated o/n at 4°C with a 1/1000 dilution .The band size is ~17 kDa (Ref.6,7) .
Tested Applications
FC, IA, IF, IHC-Fr, WB
Preservative
1 ml (> 200 μg/ml) culture medium with a low endotoxin level containing 0.02% sodium azide.
Isotype
Mouse IgG3
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