TLR2, Human, mAb TL2.1 Antibody

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.

FC: 2 ug antibody per 300000 cells; untreated as well as 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells or use 4 % faraformaldehyde as fixative! (Ref 5) IF: HMECs were fixed with 4% paraformaldehyde in PBS, permeabilized and blocked before staining; antibody concentration used 5-10μg/ml W: peripheral blood mononuclear cells; non-reduced; 1 μg/ml antibody; size ~90 kDa F: fixed in acetone; 3 % H2O2 for blockade of endogenous peroxidases; human tonsils as positive control (useful, but clone TL2.3 is preferred; Ref 2) P: HOPE-fixed; alveolar epithelial cells type II in human lung as positive control; 1 μg/ml antibody for 16 hr at 4 °C (Ref 4) IP: lysed monocytes were immunoprecipitated with antibody-conjugated Sepharose; size ~90 kDa (Ref 2) FS: 1 ug/5x107 cells; antibody blocks TNFa release (induced by bacteria) from peripheral blood mononuclear cells (Ref 1) .