JAM-A, Human, mAb M.Ab.F11 Antibody
Mouse monoclonal antibody to JAM-A
Product Specifications
Product Name Alternative
JAM-1, platelet F11 receptor, F11R, platelet adhesion molecule, CD321
UniProt
Q9Y624
Reactivity
Human
Cross Reactivity
Mouse
Immunogen
Human platelet membranes
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Cardiovascular Research; Immunology & Inflammation
Dilution
For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user's experimental setting.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
FC: Antibody M.Ab. F11 stains the extracellular domain of JAM-A protein name. Cells were incubated with 5 μg/ml of mAb in 0.1%BSA/PBS. As negative control an isotype-matched antibody was used (Ref.4) W: A non-reduced and reduced sample treatment and SDS-Page was used. The band sizes- are 32 and 35kDa (Ref. 1) . FS: Antibody M.Ab. F11 functions as anagonist resulting in platelet aggregation at concentrations of approximately 5 μg/ml (Ref.1) .
Tested Applications
FC, Functional Studies, IA, IF, IP, PS, WB
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG1
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