EPCR, Human, mAb RCR-252 Antibody
Mouse monoclonal antibody to EPCR
Product Specifications
Product Name Alternative
Activated protein C receptor, CD201, Endothelial cell protein C receptor Gene name: PROCR
Reactivity
Human
Immunogen
Human EPCR-positive RE-1 cells
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Cardiovascular Research
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
FC: Antibody RCR-252 stains the extracellular domain of human EPCR. As positive control RE-1 cells were used and as negative control N1 cells. (Ref.1) W: A non-reduced sample treatment was used. The observed band size is 50 kDa (Ref.5) . FS: Antibody RCR-252 functions as blocking antbody. It ablates APC binding to EPCR and blocks APC-mediated signaling. The antibody was functionally tested by pretreatment of cells with 10-25 μg/ml RCR-252 (Ref.1-4) .
Tested Applications
FC, Functional Studies, IP, WB
Preservative
1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum.
Isotype
Rat IgG1
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